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Revision as of 16:02, 22 June 2011 by Curtish@uab.edu (Talk | contribs)

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Load and Link approach

transfer and uncompress (slow)

  1. login to cheaha.uabgrid.uab.edu (linux),
  2. create directory for this data set in your scratch dir
    1. mkdir /lustre/scratch/user/proj1
    2. make sure that directory is readable by galaxy user
      1. Chmod og+x /lustre/scratch/user
      2. Chmod og+x /lustre/scratch/user/proj1
  3. transfer the files with SCP
    1. I used "Secure Shell Client" for windows, available at UABIT
  4. UNCOMPRESS fastq.gz files!!
    1. cd /lustre/scratch/user/proj1
      1. find `pwd` -name "*.gz" -exec ksh -c 'qrsh "gzip -d \{}" &' \;
      2. ls -1 *.gz | xargs -L 1 -i_f_ ksh -c 'qrsh -cwd gzip -d _f_ &' \;
      3. gzip -d filename
  5. make sure the files are readable by galaxy user
    1. Chmod og+r /lustre/scratch/user/proj1/*

link into galaxy dataset (fast)

  1. get Admin privileges on galaxy
    1. either get Shantanu to make you admin in Galaxy
    2. or grab someone who is (John, Curtis)
  2. In Galaxy GUI:
    1. admin > Manage Data Libraries > create new library
      1. add Datasets
        1. Upload option: Upload files from system path
          1. Using ssh run {find `pwd`} in /lustrate/scratch/user/proj1/*
          2. paste list of absolute path names into URL/Text box in Web Admin GUI
        2. Change "Copy data into Galaxy?" to "Link to files without copying into Galaxy"
        3. Put something mnemonic in Message box.

link data into a history (fast)

I could then select the datasets and, at bottom of page "For selected datasets: <Import to histories>" and get them into a history so I can compute on them.

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