FreeSurfer

FreeSurfer is a freely available software package developed by investigators at the Athinoula A. Martinos Center for Biomedical Imaging used for a number of procedures including:

1. Creation of computerized models of the brain from magnetic resonance imaging (MRI) data.
2. Processing of functional magnetic resonance imaging (fMRI) data.
3. Measuring a number of morphometric properties of the brain including cortical thickness and regional volumes.
4. Intersubject averaging of structural and functional data using a procedure that aligns individuals based on their cortical folding patterns for optimal alignment of homologous neural regions.

Steps to run FreeSurfer

Once you log into Cheaha, set up your environment by copying the following to your $HOME/.bashrc:

export FREESURFER_HOME=/share/apps/freesurfer/4.5
source $FREESURFER_HOME/SetUpFreeSurfer.sh
export FSFAST_HOME=$FREESURFER_HOME/fsfast
export FSF_OUTPUT_FORMAT=nii
export MNI_DIR=$FREESURFER_HOME/mni
export FSL_DIR=$HOME/fsl
export PATH=$FREESURFER_HOME/bin:$FSFAST_HOME/bin:$MNI_DIR/bin:$PATH

Source the above environment variables. Only needed to continue this tutorial. Need not source env variables defined in .bashrc from the next time you login to Cheaha.

source $HOME/.bashrc

FreeSurfer run its process in a non-parallel environment, so you won't have benefit from a dual/quad/hex core machine for a single case analysis. However you can start a number of FreeSurfer recon-all process in the same machine and theoretically you can reduce the time to analyze your group of cases.

This is a simple SGE script to submit recon-all process. This script first copies your subjects directory form your $HOME containing the MRI data to Cheaha's high performance file system, $UABGRID_SCRATCH, so as to ramp the speed of recon-all process. The recon-all process is then executed on $UABGRID_SCRATCH.

NOTE: Modify the SUBJECTS_DIR location to your local settings

#!/bin/bash

function run_recon-all() {

for patient in `ls -1`
do
unset SUBJECTS_DIR
SUBJECTS_DIR=$UABGRID_SCRATCH/subjects
export SUBJECTS_DIR
cat > submit_recon-all-$patient.sh <<EOF 
#!/bin/bash
#$ -S/bin/bash
#$ -cwd
#
#$ -N recon-all-job$patient
# Set the hard and soft run time limits (ex: 1hour/58 min)
#$ -l h_rt=30:00:00,s_rt=29:55:00
#$ -j y
#
#$ -M YOUR_EMAIL_ADDRESS
#$ -m eas
# Load the appropriate module file(s) for your job

mri_convert -oi -os $SUBJECTS_DIR/$patient.nii $SUBJECTS_DIR/$patient.mgz 

sleep 15

recon-all -sd $SUBJECTS_DIR -s $patient
EOF

qsub submit_recon-all-$patient.sh

done

}

cp -r $HOME/subjects $UABGRID_SCRATCH/

cd $UABGRID_SCRATCH/subjects

run_recon-all

Make the above script executable

chmod +x <name-of-script-above>

Then submit that script with

./name-of-script-above

Check on it with qstat.

qstat -u $USER

After the completion of the recon-all process, copy the results back to your $HOME and delete the contents from scratch

cp -r $UABGRID_SCRATCH/subjects $HOME/subjects-results

rm -rf $UABGRID_SCRATCH/subjects